Rapamycin response network
In this page we present the modules, and background information
for the rapamycin response modules.
We selected 14 transcriptional regulators that seemed likely to function in
the rapamycin response in S. cerevisiae, and performed genome-wide protein-DNA
binding assays. In the the following table we
present these 14 factors, and discuss why each of them were selected. You
can access the complete binding results for rapamycin by following this
link.
We ran the GRAM algorithm using the binding data for the 14 transcription
factors in rapamycin and 22 previously published expression experiments
relevant to rapamycin conditions (see [1,2]).
Thirty-nine modules containing 317 unique genes and regulated by 13
transcription factors were discovered. Click
here to download the set of rapamycin modules
identified by our algorithm. You can also view a
html version
of the same file. This version contains links for each of the orfs to its
website on SGD.
References
[1] Hardwick, J. S., Kuruvilla, F. J. Tong, J. K. Shamji, A. F. & Schreiber S. L.
Rapamycin-modulated transcription defines the subset of nutrient-sensitive signaling pathways directly controlled by the Tor proteins.
Proc. Natl. Acad. Sci. USA, 96(26), (1999).
[2] Natarajan, K. et al
Transcriptional profiling shows that Gcn4p is a master regulator of gene expression during amino acid starvation in yeast.
Mol Cell Biol. 21(13), 4347-68 (2001).